Details

Autor(en) / Beteiligte

• Christophe, Thierry
• Karlsson, Anna
• Dugave, Christophe
• Rabiet, Marie-Josèphe
• Boulay, Francois
• Dahlgren, Claes

Titel

The Synthetic Peptide Trp-Lys-Tyr-Met-Val-Met-NH2 Specifically Activates Neutrophils through FPRL1/Lipoxin A4 Receptors and Is an Agonist for the Orphan Monocyte-expressed Chemoattractant Receptor FPRL2

Ist Teil von

• The Journal of biological chemistry, 2001-06, Vol.276 (24), p.21585-21593

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2001

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Neutrophils express the G protein-coupled N-formyl peptide receptor (FPR) and its homologue FPRL1, whereas monocytes express FPR, FPRL1, and FPRL2, an orphan receptor sharing 83% amino acid identity with FPRL1. FPRL1 is a promiscuous receptor activated by serum amyloid A and by different synthetic peptides, including the hexapeptide Trp-Lys-Tyr-Met-Val-d-Met-NH2 (WKYMVm). By measuring calcium flux in HL-60 cells transfected with FPR, FPRL1, or FPRL2, we show that WKYMVm activated all three receptors, whereas thel-conformer WKYMVM activated exclusively FPRL1 and FPRL2. The functionality of FPRL2 was further assessed by the ability of HL-60-FPRL2 cells to migrate toward nanomolar concentrations of hexapeptides. The half-maximal effective concentrations of WKYMVM for calcium mobilization in HL-60-FPRL1 and HL-60-FPRL2 cells were 2 and 80 nm, respectively. Those of WKYMVm were 75 pmand 3 nm. The tritiated peptide WK[3,5-3H2]YMVM bound to FPRL1 (KD ∼ 160 nm), but not to FPR. The two conformers similarly inhibited binding of 125I-labeled WKYMVm to FPRL2-expressing cells (IC50 ∼ 2.5–3 μm). Metabolic labeling with orthophosphoric acid revealed that FPRL1 was differentially phosphorylated upon addition of the l- or d-conformer, indicating that it induced different conformational changes. In contrast to FPRL1, FPRL2 was already phosphorylated in the absence of agonist and not evenly distributed in the plasma membrane of unstimulated cells. However, both receptors were internalized upon addition of either of the two conformers. Taken together, the results indicate that neutrophils are activated by WKYMVM through FPRL1 and that FPRL2 is a chemotactic receptor transducing signals in myeloid cells.