Details

Autor(en) / Beteiligte

• He, Yue
• Chen, Ke-Yue
• Wang, Ting-Ting
• Jia, Mao
• Bai, Li-Hua
• Wang, Xiao
• Bu, Yu-Yu
• Ao, Jin-Ping

Titel

MiRNA-155 Biosensors Based on AlGaN/GaN Heterojunction Field Effect Transistors With an Au-SH-RNA Probe Gate

Ist Teil von

• IEEE transactions on electron devices, 2023-04, Vol.70 (4), p.1860-1864

Ort / Verlag

New York: IEEE

Erscheinungsjahr

2023

Link zum Volltext

• IEEE_Xplore

Quelle

IEEE Electronic Library (IEL)

Beschreibungen/Notizen

• AlGaN/gallium nitride (GaN) heterojunction field effect transistors (HFETs) have been researched widely in the fields of gas detection and ions detection. In this article, we develop a biosensor based on AlGaN/GaN HFETs to detect the tumor biomarker miRNA-155 directly. By fixing the specific sulfhydryl modified ribonucleic acid (SH-RNA) probe on the Au-gate surface of the AlGaN/GaN HFET to fabricate an RNA-Au-AlGaN/GaN HFET biosensor, this biosensor can conveniently and rapidly detect miRNA-155. The experimental results show that this biosensor has an excellent detection limit of 1.81 fM and a high sensitivity of <inline-formula> <tex-math notation="LaTeX">77\,\mu \text{A} </tex-math></inline-formula>/log concentration with a linear range (2 fM-2 nM). The limit of detection is the lowest among the reported AlGaN/GaN HFET RNA biosensors. Meanwhile, this biosensor shows a high specific recognition capacity but is weak sensitive to the mismatched miRNA.