Details

Autor(en) / Beteiligte

• Reimer, Katharina C.
• Nagai, James S.
• Varghese, Finny S.
• Overheul, Gijs J.
• Roverts, Rona
• Willemsen, Brigith
• Beukenboom, Marcel
• Dunnen, Wilfred den
• Triana, Sergio H.
• Timm, Marie-Cherelle
• van den Berge, Bartholomeus T.
• Nlandu, Quincy
• Heijnert, Joelle
• Bindels, Eric M.J.
• Hoogenboezem, Remco M.
• Mooren, Fieke
• Kuppe, Christoph
• Grünberg, Katrien
• Steenbergen, Eric J.
• Czogalla, Jan
• Sommerdijk, Nico
• Akiva, Anat
• Boor, Peter
• Huber, Tobias B.
• Bar-David, Elad
• Barr, Haim
• Boby, Melissa L.
• Borden, Bruce
• Bowman, Gregory R.
• Brun, Juliane
• BVNBS, Sarma
• Carbery, Anna
• Chernychenko, Eugene
• Clyde, Austin
• Coffland, Joseph E.
• Cole, Jason
• Cox, Lisa
• Dudgeon, Tim
• Dunnett, Louise
• Erez, Noam
• Fate, Gwen
• Ferrins, Lori
• Foster, Richard
• Foster, Holly
• Gabizon, Ronen
• Gawriljuk, Victor O.
• Gehrtz, Paul
• Gileadi, Carina
• Giroud, Charline
• Glen, Robert
• Gorichko, Marian
• Griffen, Ed J.
• Heer, Jag
• Hill, Michelle
• Horrell, Sam
• Jajack, Andrew
• De Jonghe, Steven
• Kantsadi, Anastassia L.
• Koekemoer, Lizbe
• Lee, Alpha A.
• Lefker, Bruce A.
• Macdonald, Hannah Bruce
• Maclean, Beth
• McCorkindale, Willam
• McGovern, Briana L.
• Michurin, Oleg
• Morris, Aaron
• Morris, Garret M.
• Neyts, Johan
• Oliva, Glaucius
• Owen, David
• Psenak, Vladimir
• Pai, Ruby
• Paran, Nir
• Perry, Benjamin
• Pingle, Maneesh
• Politi, Boaz
• Powell, Ailsa
• Puni, Reut
• Resnick, Efrat
• Rosales, Romel
• Schofield, Chris
• Shafeev, Mikhail
• Sittner, Assa
• Skyner, Rachael
• Spencer, John
• Tamir, Hadas
• Tennant, Rachael
• Tumber, Anthony
• van Rij, Ronald P.
• Voelz, Vincent
• Volkamer, Andra
• von Delft, Annette
• White, Kris M.
• Wright, Nathan
• Zidane, Hadeer
• Costa, Ivan G.
• Schneider, Rebekka K.
• Smeets, Bart

Titel

SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids

Ist Teil von

• Cell stem cell, 2022-02, Vol.29 (2), p.217-231.e8

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Kidney failure is frequently observed during and after COVID-19, but it remains elusive whether this is a direct effect of the virus. Here, we report that SARS-CoV-2 directly infects kidney cells and is associated with increased tubule-interstitial kidney fibrosis in patient autopsy samples. To study direct effects of the virus on the kidney independent of systemic effects of COVID-19, we infected human-induced pluripotent stem-cell-derived kidney organoids with SARS-CoV-2. Single-cell RNA sequencing indicated injury and dedifferentiation of infected cells with activation of profibrotic signaling pathways. Importantly, SARS-CoV-2 infection also led to increased collagen 1 protein expression in organoids. A SARS-CoV-2 protease inhibitor was able to ameliorate the infection of kidney cells by SARS-CoV-2. Our results suggest that SARS-CoV-2 can directly infect kidney cells and induce cell injury with subsequent fibrosis. These data could explain both acute kidney injury in COVID-19 patients and the development of chronic kidney disease in long COVID. [Display omitted] •COVID-19 patients present tubulo-interstitial kidney fibrosis compared with controls•SARS-CoV-2 infection stimulates profibrotic signaling in human kidney organoids•SARS-CoV-2 infection can be inhibited by a protease blocker in human kidney organoids Jansen, Reimer, Nagai, et al. report that SARS-CoV-2 infects kidney cells and is associated with kidney fibrosis in patients. Using single-cell transcriptomics of infected kidney organoids, they show that SARS-CoV-2 causes kidney injury and stimulates profibrotic signaling. Viral infection in organoids was inhibited by a recently developed protease blocker.