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Determination of Estrogenic Activity in Beer by Biological and Chemical Means
Ist Teil von
Journal of agricultural and food chemistry, 2001-02, Vol.49 (2), p.633-640
Ort / Verlag
Washington, DC: American Chemical Society
Erscheinungsjahr
2001
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
It has been suspected that beer drinking may change the hormonal status of men caused by phytoestrogens. Five different Austrian lager beers have been investigated for estrogenic activity by a yeast two-plasmid system harboring the human estrogen receptor α, after concentration by solid phase extraction. The beer concentrate was further fractionated by reversed phase HPLC, and then the fractions were characterized by the biological assay and GC-MS. The most potent fraction did not contain a known phytoestrogen. The total activity corresponded to an average of 43 ng of 17β-estradiol/L of beer. It was concluded that the human health hazard of beer drinking originating from compounds activity on the estrogen receptor α is negligible. Keywords: Phytoestrogens; beer; yeast; isoflavonoids