Details

Autor(en) / Beteiligte

• Salek, Mogjiborahman
• Förster, Jonas D.
• Lehmann, Wolf-Dieter
• Riemer, Angelika B.

Titel

Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics

Ist Teil von

• Analytical and bioanalytical chemistry, 2022-03, Vol.414 (8), p.2545-2552

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2022

Link zum Volltext

Quelle

SpringerNature Journals

Beschreibungen/Notizen

• In mass spectrometry-based proteomics, heavy internal standards are used to validate target peptide detections and to calibrate peptide quantitation. Here, we report light contamination present in heavy labelled synthetic peptides of high isotopic enrichment. Application of such peptides as assay-internal standards potentially compromises the detection and quantitation especially of low abundant cellular peptides. Therefore, it is important to adopt guidelines to prevent false-positive identifications of endogenous light peptides as well as errors in their quantitation from biological samples.