Details

Autor(en) / Beteiligte

• Meier, Doreen
• Kruse, Janis
• Buttlar, Jann
• Friedrich, Michael
• Zenk, Fides
• Boesler, Benjamin
• Förstner, Konrad U
• Hammann, Christian
• Nellen, Wolfgang
• Chen, Xuemei

Titel

Analysis of the Microprocessor in Dictyostelium: The Role of RbdB, a dsRNA Binding Protein

Ist Teil von

• PLoS genetics, 2016-06, Vol.12 (6), p.e1006057-e1006057

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2016

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• We identified the dsRNA binding protein RbdB as an essential component in miRNA processing in Dictyostelium discoideum. RbdB is a nuclear protein that accumulates, together with Dicer B, in nucleolar foci reminiscent of plant dicing bodies. Disruption of rbdB results in loss of miRNAs and accumulation of primary miRNAs. The phenotype can be rescued by ectopic expression of RbdB thus allowing for a detailed analysis of domain function. The lack of cytoplasmic dsRBD proteins involved in miRNA processing, suggests that both processing steps take place in the nucleus thus resembling the plant pathway. However, we also find features e.g. in the domain structure of Dicer which suggest similarities to animals. Reduction of miRNAs in the rbdB- strain and their increase in the Argonaute A knock out allowed the definition of new miRNAs one of which appears to belong to a new non-canonical class.