Details

Autor(en) / Beteiligte

• Choi, Yong Jin
• Lin, Chao-Po
• Risso, Davide
• Chen, Sean
• Kim, Thomas Aquinas
• Tan, Meng How
• Li, Jin Billy
• Wu, Yalei
• Chen, Caifu
• Xuan, Zhenyu
• Macfarlan, Todd
• Peng, Weiqun
• Lloyd, K. C. Kent
• Kim, Sang Yong
• Speed, Terence P.
• He, Lin

Titel

Deficiency of microRNA miR-34a expands cell fate potential in pluripotent stem cells

Ist Teil von

• Science (American Association for the Advancement of Science), 2017-02, Vol.355 (6325), p.596-596

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2017

Link zum Volltext

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) efficiently generate all embryonic cell lineages but rarely generate extraembryonic cell types. We found that microRNA deficiency expands the developmental potential of mouse pluripotent stem cells, yielding both embryonic and extraembryonic lineages and strongly inducing MuERV-L (MERVL) endogenous retroviruses, similar to what is seen with features of totipotent two-cell blastomeres. restricts the acquisition of expanded cell fate potential in pluripotent stem cells, and it represses MERVL expression through transcriptional regulation, at least in part by targeting the transcription factor Gata2. Our studies reveal a complex molecular network that defines and restricts pluripotent developmental potential in cultured ESCs and iPSCs.