Details

Autor(en) / Beteiligte

• Taufek, Norhidayah Mohd
• Harith, Hanis H.
• Abd Rahim, Muhamad Hafiz
• Ilham, Zul
• Rowan, Neil
• Wan-Mohtar, Wan Abd Al Qadr Imad

Titel

Performance of mycelial biomass and exopolysaccharide from Malaysian Ganoderma lucidum for the fungivore red hybrid Tilapia (Oreochromis sp.) in Zebrafish embryo

Ist Teil von

• Aquaculture reports, 2020-07, Vol.17, p.100322, Article 100322

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Mycelial extracts of Ganoderma lucidum were fed to red hybrid Tilapia.•Toxicity of mycelial extracts of G. lucidum was evaluated in Zebrafish model.•Biomass extract (MB) was safe to Zebrafish hatching and embryo at 2000 μg/mL.•Exopolysaccharide (EPS) was safe for Zebrafish hatching and embryo at 3000 μg/mL.•MB and EPS of G. lucidum extracts were considered as non-toxic for vertebrates. Natural mycelial biomass (MB) and extracted exopolysaccharide (EPS) from the pre-grown Malaysian Ganoderma lucidum mushroom are both considered as high-end materials due to their high commercial value in the aquaculture industry. To evaluate their potential toxicity as a fish-feed supplement for the fungivore red hybrid Tilapia (Oreochromis sp.), both MB (250−5000 μg/mL) and EPS (62.5−3000 μg/mL) were subjected to zebrafish embryo toxicity (ZFET) assay, and the effects on zebrafish embryos (ZE) early development were analyzed between 24−120 hours of post-exposure (HPE). MB and EPS showed no toxic effect towards the ZE with LC50 of 1650 μg/mL and 2648.38 μg/mL, respectively. MB at concentrations between 250−5000 μg/mL and EPS at 3000 μg/mL showed no significant changes in ZE hatching. No significant changes in the ZE heart rate were detected following treatment with both tested compounds (MB: 250−2000 μg/mL and EPS: 62.5−3000 μg/mL) as compared to untreated embryos (135.5 beats/min). Furthermore, teratogenic effects of both MB and EPS (<3000 μg/mL) on zebrafish embryonic development were not observed. Together, both natural compounds MB and EPS can be considered non-toxic, suggesting that these can be safely applied as feed substances in the fish-feed aquaculture industry.