Details

Autor(en) / Beteiligte

• Hu, Zhicheng
• Kopparapu, Pradeep Kumar
• Ebner, Patrick
• Mohammad, Majd
• Lind, Simon
• Jarneborn, Anders
• Dahlgren, Claes
• Schultz, Michelle
• Deshmukh, Meghshree
• Pullerits, Rille
• Nega, Mulugeta
• Nguyen, Minh-Thu
• Fei, Ying
• Forsman, Huamei
• Götz, Friedrich
• Jin, Tao

Titel

Phenol-soluble modulin α and β display divergent roles in mice with staphylococcal septic arthritis

Ist Teil von

• Communications biology, 2022-09, Vol.5 (1), p.910-910, Article 910

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Abstract Phenol-soluble modulin α (PSMα) is identified as potent virulence factors in Staphylococcus aureus ( S. aureus ) infections. Very little is known about the role of PSMβ which belongs to the same toxin family. Here we compared the role of PSMs in S. aureus -induced septic arthritis in a murine model using three isogenic S. aureus strains differing in the expression of PSMs (Newman, Δ psmα , and Δ psmβ ). The effects of PSMs on neutrophil NADPH-oxidase activity were determined in vitro. We show that the PSMα activates neutrophils via the formyl peptide receptor (FPR) 2 and reduces their NADPH-oxidase activity in response to the phorbol ester PMA. Despite being a poor neutrophil activator, PSMβ has the ability to reduce the neutrophil activating effect of PSMα and to partly reverse the effect of PSMα on the neutrophil response to PMA. Mice infected with S. aureus lacking PSMα had better weight development and lower bacterial burden in the kidneys compared to mice infected with the parental strain, whereas mice infected with bacteria lacking PSMβ strain developed more severe septic arthritis accompanied with higher IL-6 and KC. We conclude that PSMα and PSMβ play distinct roles in septic arthritis: PSMα aggravates systemic infection, whereas PSMβ protects arthritis development.