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Autor(en) / Beteiligte

• Knight, Alex

Titel

Single molecule biology [electronic resource]

Link zum Volltext

• Elsevier Books AutoHoldings

Beschreibungen/Notizen

• Description based upon print version of record.
• Includes bibliographical references and index.
• Front Cover; Single Molecule Biology; Copyright Page; Contents; Preface; Acknowledgments; List of Contributors; Introduction: The ""Single Molecule"" Paradigm; The "Single Molecule" Paradigm; Life as a Molecule; Single Molecule Techniques; Overview of Single Molecule Biology; Conclusions; Acknowledgments; References; Chapter 1 Single Molecule Studies of Myosins; Introduction; Motility Assays; Molecular Mechanics; Technologies; The Myosin Working Stroke; Force and Stiffness; Kinetics; Conclusions; Acknowledgments; References
• Chapter 2 Single Molecule Experiments and the Kinesin Motor Protein Superfamily: Walking Hand in HandIntroduction; Overall Mechanical Parameters of Kinesins; Advanced Mechanochemistry of Kinesin-1; Lattice Diffusion as an Additional Motility Mode; Regulation of Kinesin Motors; Final Thoughts; Acknowledgments; References; Chapter 3 Force-Generating Mechanisms of Dynein Revealed through Single molecule Studies; Introduction; Molecular Organization of Dynein; Mechanism of Force Generation by Dyneins; Mechanical Properties of Dyneins Studied by Single molecule Methods; Dyneins in Axonemes
• Perspectives: From Single Molecules to EnsemblesAcknowledgments; References; Chapter 4 The Bacterial Flagellar Motor; Introduction; Structure; Function; Outlook; References; Chapter 5 Single Molecule Studies of Chromatin Structure and Dynamics; Introduction; Sperm Chromatin; Spermiogenesis; Previous Studies of Toroid Structure; Single molecule Experiments; The Protamines P1 and P2; The Transition Proteins TP1 and TP2; Shaping of the Sperm Head and the Role of the Manchette; Posttranscriptional Modifications of Sperm Nuclear Proteins; Conclusions: Sperm Chromatin; Somatic Chromatin; Conclusion
• AcknowledgmentsReferences; Chapter 6 Single Molecule Studies of Nucleic Acid Enzymes; Introduction; Methods; Single molecule Studies of Nucleic Acid Enzymes; Conclusion; References; Chapter 7 Single Molecule Studies of Prokaryotic Translation; Introduction; Directly Observing Translation; Translation at Atomic Resolution; Single molecule Translation; Conclusions; References; Chapter 8 Single Ion Channels; Comparison of Fluorescence Methods with Single-Channel Recording; How to Get Information about Mechanisms from Single molecule Measurements; Some Recent Results from Single-Channel Recording
• ConclusionsReferences; Chapter 9 Single Molecule Fluorescence in Membrane Biology; The Structure and Function of Cellular Membranes; Why Apply Single molecule Fluorescence to Membranes?; Fluorescent Labels and Artificial Cell Membranes; Single molecule Fluorescence Techniques; The Role of SMF in Studying Membranes and Membrane Proteins; Probing the Structure and Behavior of Cell Membranes; Membrane-Cytoskeleton Interactions; Lipid Rafts; Understanding the Dynamics and Interactions of Membrane Proteins; Signalling; Membrane Transport; Vesicle Tracking, Docking, and Fusion; Conclusions
• References
• Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, expli
• English
• Description based on publisher supplied metadata and other sources.