Details

Autor(en) / Beteiligte

• de Jesus, Liana Inara
• Smiderle, Fhernanda R.
• Cordeiro, Lucimara M.C.
• de Freitas, Rilton A.
• Van Griensven, Leo J.L.D.
• Iacomini, Marcello

Titel

Simple and effective purification approach to dissociate mixed water-insoluble α- and β-D-glucans and its application on the medicinal mushroom Fomitopsis betulina

Ist Teil von

• Carbohydrate polymers, 2018-11, Vol.200, p.353-360

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• •A mixture of water-insoluble glucans was isolated from fruiting bodies of Piptoporus betulinus.•These glucans were purified based on their different solubility in diluted aqueous NaOH solution.•The insoluble glucan was defined as a linear (1→3)-α-D-glucan.•The soluble glucan was defined as a branched (1→3)(1→6)-β-D-glucan. Differences in anomericity and in the branching degree of glucans lead to characteristic intermolecular association that influences their solubility in water or other solvents. A simple purification approach, based on the glucan solubility in aq. 0.1 M NaOH solution, was applied for the separation of mixed water-insoluble α-D-glucans from β-D-glucans extracted from fruiting bodies of Fomitopsis betulina, which is an underexploited medicinal mushroom. The results indicated that the β-D-glucan is constituted by (1→3)-linked β-D-Glcp units substituted at O-6 by non-reducing β-D-Glcp and (1→6)-linked β-D-Glcp units, while the α-D-glucan has a linear (1→3)-linked glucan structure. Thus, the 0.1 M NaOH treatment proved to be a simple, efficient and low-cost purification method for separation of water-insoluble glucans with different anomeric configurations and degree of branching that were interacting by intermolecular forces.