Details

Autor(en) / Beteiligte

• Paulson, Lars
• Narayanasamy, Sankar Raju
• Shelby, Megan L.
• Frank, Matthias
• Trebbin, Martin

Titel

Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers

Ist Teil von

• Structural dynamics (Melville, N.Y.), 2024-01, Vol.11 (1), p.011101-011101

Ort / Verlag

United States: American Institute of Physics, Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Serial crystallography at large facilities, such as x-ray free-electron lasers and synchrotrons, evolved as a powerful method for the high-resolution structural investigation of proteins that are critical for human health, thus advancing drug discovery and novel therapies. However, a critical barrier to successful serial crystallography experiments lies in the efficient handling of the protein microcrystals and solutions at microscales. Microfluidics are the obvious approach for any high-throughput, nano-to-microliter sample handling, that also requires design flexibility and rapid prototyping to deal with the variable shapes, sizes, and density of crystals. Here, we discuss recent advances in polymer 3D printing for microfluidics-based serial crystallography research and present a demonstration of emerging, large-scale, nano-3D printing approaches leading into the future of 3D sample environment and delivery device fabrication from liquid jet gas-dynamic virtual nozzles devices to fixed-target sample environment technology.