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Acyl azide modification of the ubiquitin C-terminus enables DUB capture
Ist Teil von
Chemical communications (Cambridge, England), 2023-01, Vol.59 (1), p.1333-1336
Ort / Verlag
England: Royal Society of Chemistry
Erscheinungsjahr
2023
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Deubiquitinating enzyme (DUB) abnormalities are associated with many diseases. Previous attempts have been made to introduce various chemical groups such as alkynes, unsaturated olefins and alkyl halides to the C-terminus of ubiquitin (Ub) to capture the active-site cysteine residue in DUBs for structural and biochemical studies. Here, we find that a Ub C-terminal acyl azide can capture DUBs, thereby forming thioester bonds in buffers and cell lysates. This finding not only makes ubiquitin acyl azide a chemical probe for capturing DUBs, but also extends the utility of azide groups in biological applications.
A Ub C-terminal acyl azide was synthesized and found to be able to capture DUBs.