Details

Autor(en) / Beteiligte

• Sassi, Mouna Ben
• Ferjani, Sana
• Mkada, Imen
• Arbi, Marwa
• Safer, Mouna
• Elmoussi, Awatef
• Abid, Salma
• Souiai, Oussema
• Gharbi, Alya
• Tejouri, Asma
• Gaies, Emna
• Eljabri, Hanene
• Ayed, Samia
• Hechaichi, Aicha
• Daghfous, Riadh
• Gouider, Riadh
• Khelil, Jalila Ben
• Kharrat, Maher
• Kacem, Imen
• Alya, Nissaf Ben
• Benkahla, Alia
• Trabelsi, Sameh
• Boubaker, Ilhem Boutiba-Ben

Titel

Phylogenetic and amino acid signature analysis of the SARS-CoV-2s lineages circulating in Tunisia

Ist Teil von

• Infection, genetics and evolution, 2022-08, Vol.102, p.105300-105300, Article 105300

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Since the beginning of the Coronavirus disease-2019 pandemic, there has been a growing interest in exploring SARS-CoV-2 genetic variation to understand the origin and spread of the pandemic, improve diagnostic methods and develop the appropriate vaccines. The objective of this study was to identify the SARS-CoV-2s lineages circulating in Tunisia and to explore their amino acid signature in order to follow their genome dynamics. Whole genome sequencing and genetic analyses of fifty-eight SARS-CoV-2 samples collected during one-year between March 2020 and March 2021 from the National Influenza Center were performed using three sampling strategies.. Multiple lineage introductions were noted during the initial phase of the pandemic, including B.4, B.1.1, B.1.428.2, B.1.540 and B.1.1.189. Subsequently, lineages B1.160 (24.2%) and B1.177 (22.4%) were dominant throughout the year. The Alpha variant (B.1.1.7 lineage) was identified in February 2021 and firstly observed in the center of our country. In addition, A clear diversity of lineages was observed in the North of the country. A total of 335 mutations including 10 deletions were found. The SARS-CoV-2 proteins ORF1ab, Spike, ORF3a, and Nucleocapsid were observed as mutation hotspots with a mutation frequency exceeding 20%. The 2 most frequent mutations, D614G in S protein and P314L in Nsp12 appeared simultaneously and are often associated with increased viral infectivity. Interestingly, deletions in coding regions causing consequent deletions of amino acids and frame shifts were identified in NSP3, NSP6, S, E, ORF7a, ORF8 and N proteins. These findings contribute to define the COVID-19 outbreak in Tunisia. Despite the country's limited resources, surveillance of SARS-CoV-2 genomic variation should be continued to control the occurrence of new variants. •One year of SARS-CoV-2 genomic surveillance in Tunisia.•Multiple lineage introductions were noted during the early stage of the pandemic•Clear diversity of lineages in the North of the country.•SARS-CoV-2 ORF1ab, spike, ORF3a, and nucleocapsid proteins: mutational hotspots with mutation frequency exceeding 20%.•Deletions of amino acids and frameshifts were identified in NSP3, NSP6, protein S, protein E, ORF7a, ORF8 and protein N.