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A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex
Nature (London), 2021-10, Vol.598 (7879), p.103-110
2021
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Autor(en) / Beteiligte
Titel
A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex
Ist Teil von
  • Nature (London), 2021-10, Vol.598 (7879), p.103-110
Ort / Verlag
England: Nature Publishing Group
Erscheinungsjahr
2021
Quelle
MEDLINE
Beschreibungen/Notizen
  • Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain . With the proliferation of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of cell-type organization. Here we generated transcriptomes and epigenomes from more than 500,000 individual cells in the mouse primary motor cortex, a structure that has an evolutionarily conserved role in locomotion. We developed computational and statistical methods to integrate multimodal data and quantitatively validate cell-type reproducibility. The resulting reference atlas-containing over 56 neuronal cell types that are highly replicable across analysis methods, sequencing technologies and modalities-is a comprehensive molecular and genomic account of the diverse neuronal and non-neuronal cell types in the mouse primary motor cortex. The atlas includes a population of excitatory neurons that resemble pyramidal cells in layer 4 in other cortical regions . We further discovered thousands of concordant marker genes and gene regulatory elements for these cell types. Our results highlight the complex molecular regulation of cell types in the brain and will directly enable the design of reagents to target specific cell types in the mouse primary motor cortex for functional analysis.
Sprache
Englisch
Identifikatoren
ISSN: 0028-0836
eISSN: 1476-4687
DOI: 10.1038/s41586-021-03500-8
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8494649
Format
–
Schlagworte
Animals, Atlases as Topic, Biological effects, Computational neuroscience, Cortex (motor), Datasets, Datasets as Topic, DNA methylation, Epigenesis, Genetic, Epigenomics, Female, Functional analysis, Gene expression, Gene Expression Profiling, Locomotion, Male, Mice, Motor Cortex - anatomy & histology, Motor Cortex - cytology, Neurons - classification, Neurons - cytology, Neurons - metabolism, Organ Specificity, Pyramidal cells, Regulatory sequences, Reproducibility of Results, Single-Cell Analysis, Statistical methods, Taxonomy, Transcriptome, Transcriptomes

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