Details

Autor(en) / Beteiligte

• Schwartz, Steven H
• Hendrix, Bill
• Hoffer, Paul
• Sanders, Rick A
• Zheng, Wei

Titel

Carbon Dots for Efficient Small Interfering RNA Delivery and Gene Silencing in Plants

Ist Teil von

• Plant physiology (Bethesda), 2020-10, Vol.184 (2), p.647-657

Ort / Verlag

United States: American Society of Plant Biologists

Erscheinungsjahr

2020

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The initiation of RNA interference (RNAi) by topically applied small interfering RNA has potential applications for plant functional genomics, crop improvement and crop protection, but the primary obstacle for the development of this technology is the efficient delivery of RNAi effectors into the cell. The plant cell wall is a particularly challenging barrier for the delivery of macromolecules because many of the transfection agents that are commonly used with animal cells produce nanocomplexes that are significantly larger than the size exclusion limit of the cell wall. Here, we illustrate the use of a class of very small nanoparticles, called carbon dots, for delivering small interfering RNA into the model plants and tomato ( ). Low-pressure spray application of these formulations with a spreading surfactant resulted in strong silencing of transgenes in both species. The delivery efficacy of carbon dot formulations was also demonstrated by the silencing of endogenous genes that encode two subunits of magnesium chelatase, an enzyme necessary for chlorophyll synthesis. The strong visible phenotypes observed with the carbon dot-facilitated delivery were validated by measuring significant reductions in the target gene transcript and/or protein levels. Methods for the delivery of RNAi effectors into plants, such as the carbon dot formulations described here, could become valuable tools for gene silencing in plants with practical applications in plant functional genomics and agriculture.