Details

Autor(en) / Beteiligte

• Xiong, Hua-Long
• Wu, Yang-Tao
• Cao, Jia-Li
• Yang, Ren
• Liu, Ying-Xia
• Ma, Jian
• Qiao, Xiao-Yang
• Yao, Xiang-Yang
• Zhang, Bao-Hui
• Zhang, Ya-Li
• Hou, Wang-Heng
• Shi, Yang
• Xu, Jing-Jing
• Zhang, Liang
• Wang, Shao-Juan
• Fu, Bao-Rong
• Yang, Ting
• Ge, Sheng-Xiang
• Zhang, Jun
• Yuan, Quan
• Huang, Bao-Ying
• Li, Zhi-Yong
• Zhang, Tian-Ying
• Xia, Ning-Shao

Titel

Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells

Ist Teil von

• Emerging microbes & infections, 2020-01, Vol.9 (1), p.2105-2113

Ort / Verlag

United States: Taylor & Francis

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based on VSV-SARS-CoV-2-Sdel18 pseudovirus and hACE2-overexpressing BHK21 cells (BHK21-hACE2 cells). The experimental results can be obtained by automatically counting the number of EGFP-positive cells at 12 h after infection, making the assay convenient and high-throughput. The serum neutralizing titer measured by the VSV-SARS-CoV-2-Sdel18 pseudovirus assay has a good correlation with that measured by the wild type SARS-CoV-2 assay. Seven neutralizing monoclonal antibodies targeting the receptor binding domain (RBD) of the SARS-CoV-2 S protein were obtained. This efficient and reliable pseudovirus assay model could facilitate the development of new drugs and vaccines.