Details

Autor(en) / Beteiligte

• Lichtmannsperger, Katharina
• Hinney, Barbara
• Joachim, Anja
• Wittek, Thomas

Titel

Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests

Ist Teil von

• Comparative immunology, microbiology and infectious diseases, 2019-10, Vol.66, p.101333-101333, Article 101333

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• [Display omitted] •Giardia intestinalis (48/177) and Cryptosporidium parvum (98/177) occur in Austrian calves.•C. parvum genotypes IIaA15G2R1, IIaA19G2R2, IIaA21G2R1 and IIaA14G1R1 were found.•Giardia intestinalis assemblage E and a single sample of assemblage A were found.•Cryptosporidium was detected mostly in the first two weeks of age (72/102).•Point-of-care test validity varied greatly for Giardia and Cryptosporidium. To obtain information about the occurrence and genotype distribution of G. intestinalis and C. parvum in Austrian cattle, faecal samples from diarrhoeic calves younger than 180 days of age originating from 70 farms were examined. Of the 177 faecal samples, 27.1% were positive for Giardia cysts (immunofluorescence microscopy) and 55.4% for Cryptosporidium oocysts (phase-contrast microscopy). Positive samples were characterized by nested PCR for Giardia, 83.3% (triosephosphate isomerase; tpi) and 89.6% (β-giardin; bg) were positive, while the Cryptosporidium nested PCR returned 92.5% (60-kDa glycoprotein) positive results. Sequence analysis revealed one assemblage A-positive sample and 30 (bg) respectively 29 (tpi) assemblage E-positive samples for G. intestinalis. For C. parvum four subtypes within the IIa family (IIaA15G2R1, n = 29; IIaA19G2R2, n = 3; IIaA21G2R1, n = 2; IIaA14G1R1, n = 1) could be differentiated. Validation of two immunochromatographic point-of-care tests resulted in a sensitivity of 29.2% and 77.6%; a specificity of 98.4% and 91.1% for the detection of Giardia intestinalis and Cryptosporidium parvum, respectively. Results confirm the widespread occurrence of both protozoa in diarrhoeic calves in Austria.