Scientific reports, 2020-01, Vol.10 (1), p.940, Article 940
2020
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- Autor(en) / Beteiligte
- Titel
- Pol μ ribonucleotide insertion opposite 8-oxodG facilitates the ligation of premutagenic DNA repair intermediate
- Ist Teil von
- Scientific reports, 2020-01, Vol.10 (1), p.940, Article 940
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- DNA polymerase (pol) μ primarily inserts ribonucleotides into a single-nucleotide gapped DNA intermediate, and the ligation step plays a critical role in the joining of noncomplementary DNA ends during nonhomologous end joining (NHEJ) for the repair of double-strand breaks (DSBs) caused by reactive oxygen species. Here, we report that the pol μ insertion products of ribonucleotides (rATP or rCTP), instead of deoxyribonucleotides, opposite 8-oxo-2'-deoxyguanosine (8-oxodG) are efficiently ligated and the presence of Mn stimulates this coupled reaction in vitro. Moreover, our results point to a role of pol μ in mediating ligation during the mutagenic bypass of 8-oxodG, while 3'-preinserted noncanonical base pairs (3'-rA or 3'-rC) on NHEJ repair intermediates compromise the end joining by DNA ligase I or the DNA ligase IV/XRCC4 complex.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-020-57886-yTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6976671
- Format
- –
- Schlagworte
- 8-Hydroxy-2'-Deoxyguanosine, Deoxyguanosine, Deoxyribonucleic acid, Deoxyribonucleotides, DNA, DNA Breaks, Double-Stranded, DNA End-Joining Repair - genetics, DNA ligase (ATP), DNA Ligase ATP - genetics, DNA Ligase ATP - metabolism, DNA repair, DNA-directed DNA polymerase, DNA-Directed DNA Polymerase - genetics, DNA-Directed DNA Polymerase - metabolism, Humans, Intermediates, Manganese, Mutagenesis, Insertional, Non-homologous end joining, Reactive Oxygen Species, Ribonucleotides, Ribonucleotides - genetics, Ribonucleotides - metabolism