Details

Autor(en) / Beteiligte

• Lukowski, Samuel W
• Lo, Camden Y
• Sharov, Alexei A
• Nguyen, Quan
• Fang, Lyujie
• Hung, Sandy SC
• Zhu, Ling
• Zhang, Ting
• Grünert, Ulrike
• Nguyen, Tu
• Senabouth, Anne
• Jabbari, Jafar S
• Welby, Emily
• Sowden, Jane C
• Waugh, Hayley S
• Mackey, Adrienne
• Pollock, Graeme
• Lamb, Trevor D
• Wang, Peng‐Yuan
• Hewitt, Alex W
• Gillies, Mark C
• Powell, Joseph E
• Wong, Raymond CB

Titel

A single‐cell transcriptome atlas of the adult human retina

Ist Teil von

• The EMBO journal, 2019-09, Vol.38 (18), p.e100811-n/a

Ort / Verlag

England: Blackwell Publishing Ltd

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• The retina is a specialized neural tissue that senses light and initiates image processing. Although the functional organization of specific retina cells has been well studied, the molecular profile of many cell types remains unclear in humans. To comprehensively profile the human retina, we performed single‐cell RNA sequencing on 20,009 cells from three donors and compiled a reference transcriptome atlas. Using unsupervised clustering analysis, we identified 18 transcriptionally distinct cell populations representing all known neural retinal cells: rod photoreceptors, cone photoreceptors, Müller glia, bipolar cells, amacrine cells, retinal ganglion cells, horizontal cells, astrocytes, and microglia. Our data captured molecular profiles for healthy and putative early degenerating rod photoreceptors, and revealed the loss of MALAT1 expression with longer post‐mortem time, which potentially suggested a novel role of MALAT1 in rod photoreceptor degeneration. We have demonstrated the use of this retina transcriptome atlas to benchmark pluripotent stem cell‐derived cone photoreceptors and an adult Müller glia cell line. This work provides an important reference with unprecedented insights into the transcriptional landscape of human retinal cells, which is fundamental to understanding retinal biology and disease. Synopsis The transcriptome of human neural retina at a single‐cell level defines the gene expression profile in major cell types in the neural retina and can be used as a benchmark to assess the quality of stem cell‐derived cells or primary retinal cells. The presented transcriptome atlas of human neural retina comprises single‐cell RNA‐sequencing data from 20,009 human retinal cells. Unsupervised cell clustering analysis allows identification of 18 transcriptionally distinct cell populations that represent all known neural retinal cell types. Reduced expression of the long non‐coding RNA MALAT1 correlates with longer post‐mortem time in putative early degenerating rod photoreceptors. The retina transcriptome atlas can be used to benchmark pluripotent stem cell‐derived cone photoreceptors and an adult Müller glia cell line. A comprehensive analysis of the human retinal transcriptome at a single‐cell level defines gene expression profiles of all major retinal cell types.