Details

Autor(en) / Beteiligte

• Schnieders, Robbin
• Wolter, Antje C.
• Richter, Christian
• Wöhnert, Jens
• Schwalbe, Harald
• Fürtig, Boris

Titel

Novel 13C‐detected NMR Experiments for the Precise Detection of RNA Structure

Ist Teil von

• Angewandte Chemie (International ed.), 2019-07, Vol.58 (27), p.9140-9144

Auflage

International ed. in English

Ort / Verlag

Weinheim: Wiley Subscription Services, Inc

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Up to now, NMR spectroscopic investigations of RNA have utilized imino proton resonances as reporters for base pairing and RNA structure. The nucleobase amino groups are often neglected, since most of their resonances are broadened beyond detection due to rotational motion around the C–NH2 bond. Here, we present 13C‐detected NMR experiments for the characterization of all RNA amino groups irrespective of their motional behavior. We have developed a C(N)H‐HDQC experiment that enables the observation of a complete set of sharp amino resonances through the detection of proton‐NH2 double quantum coherences. Further, we present an “amino”‐NOESY experiment to detect NOEs to amino protons, which are undetectable by any other conventional NOESY experiment. Together, these experiments allow the exploration of additional chemical shift information and inter‐residual proton distances important for high‐resolution RNA secondary and tertiary structure determination. In NMR spectroscopy of RNA, imino groups are usually used as a reporter for base pairing, whereas amino groups are neglected as their resonances are broadened beyond detection. 13C‐detected NMR experiments allow a full characterization of amino groups. Inevitably, amino protons can be used as a new standard for the characterization of nucleobase interactions in nucleic acids.