Details

Autor(en) / Beteiligte

• Loveridge, E Joel
• Jones, Cerith
• Bull, Matthew J
• Moody, Suzy C
• Kahl, Małgorzata W
• Khan, Zainab
• Neilson, Louis
• Tomeva, Marina
• Adams, Sarah E
• Wood, Andrew C
• Rodriguez-Martin, Daniel
• Pinel, Ingrid
• Parkhill, Julian
• Mahenthiralingam, Eshwar
• Crosby, John
• Zhulin, Igor B.

Titel

Reclassification of the Specialized Metabolite Producer Pseudomonas mesoacidophila ATCC 31433 as a Member of the Burkholderia cepacia Complex

Ist Teil von

• Journal of bacteriology, 2017-07, Vol.199 (13)

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2017

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• ATCC 31433 is a Gram-negative bacterium, first isolated from Japanese soil samples, that produces the monobactam isosulfazecin and the β-lactam-potentiating bulgecins. To characterize the biosynthetic potential of ATCC 31433, its complete genome was determined using single-molecule real-time DNA sequence analysis. The 7.8-Mb genome comprised four replicons, three chromosomal (each encoding rRNA) and one plasmid. Phylogenetic analysis demonstrated that ATCC 31433 was misclassified at the time of its deposition and is a member of the complex, most closely related to The sequenced genome shows considerable additional biosynthetic potential; known gene clusters for malleilactone, ornibactin, isosulfazecin, alkylhydroxyquinoline, and pyrrolnitrin biosynthesis and several uncharacterized biosynthetic gene clusters for polyketides, nonribosomal peptides, and other metabolites were identified. Furthermore, ATCC 31433 harbors many genes associated with environmental resilience and antibiotic resistance and was resistant to a range of antibiotics and metal ions. In summary, this bioactive strain should be designated complex strain ATCC 31433, pending further detailed taxonomic characterization. This work reports the complete genome sequence of ATCC 31433, a known producer of bioactive compounds. Large numbers of both known and novel biosynthetic gene clusters were identified, indicating that ATCC 31433 is an untapped resource for discovery of novel bioactive compounds. Phylogenetic analysis demonstrated that ATCC 31433 is in fact a member of the complex, most closely related to the species Further investigation of the classification and biosynthetic potential of ATCC 31433 is warranted.