Details

Autor(en) / Beteiligte

• Mortensen, Simon A.
• Sander, Bjoern
• Jensen, Rasmus K.
• Pedersen, Jan Skov
• Golas, Monika M.
• Jensenius, Jens C.
• Hansen, Annette G.
• Thiel, Steffen
• Andersen, Gregers R.

Titel

Structure and activation of C1, the complex initiating the classical pathway of the complement cascade

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2017-01, Vol.114 (5), p.986-991

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2017

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The complement system is an important antimicrobial and inflammation-generating component of the innate immune system. The classical pathway of complement is activated upon binding of the 774-kDa C1 complex, consisting of the recognitionmolecule C1q and the tetrameric protease complex C1r₂s₂, to a variety of activators presenting specific molecular patterns such as IgG- and IgM-containing immune complexes. A canonical model entails a C1r₂s₂ with its serine protease domains tightly packed together in the center of C1 and an intricate intramolecular reaction mechanism for activation of C1r and C1s, induced upon C1 binding to the activator. Here, we show that the serine protease domains of C1r and C1s are located at the periphery of the C1r₂s₂ tetramer both when alone or within the nonactivated C1 complex. Our structural studies indicate that the C1 complex adopts a conformation incompatible with intramolecular activation of C1, suggesting instead that intermolecular proteolytic activation between neighboring C1 complexes bound to a complement activating surface occurs. Our results rationalize how a multitude of structurally unrelated molecular patterns can activate C1 and suggests a conserved mechanism for complement activation through the classical and the related lectin pathway.