Details

Autor(en) / Beteiligte

• Dowell, Noah L.
• Giorgianni, Matt W.
• Kassner, Victoria A.
• Selegue, Jane E.
• Sanchez, Elda E.
• Carroll, Sean B.

Titel

The Deep Origin and Recent Loss of Venom Toxin Genes in Rattlesnakes

Ist Teil von

• Current biology, 2016-09, Vol.26 (18), p.2434-2445

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• The genetic origin of novel traits is a central but challenging puzzle in evolutionary biology. Among snakes, phospholipase A2 (PLA2)-related toxins have evolved in different lineages to function as potent neurotoxins, myotoxins, or hemotoxins. Here, we traced the genomic origin and evolution of PLA2 toxins by examining PLA2 gene number, organization, and expression in both neurotoxic and non-neurotoxic rattlesnakes. We found that even though most North American rattlesnakes do not produce neurotoxins, the genes of a specialized heterodimeric neurotoxin predate the origin of rattlesnakes and were present in their last common ancestor (∼22 mya). The neurotoxin genes were then deleted independently in the lineages leading to the Western Diamondback (Crotalus atrox) and Eastern Diamondback (C. adamanteus) rattlesnakes (∼6 mya), while a PLA2 myotoxin gene retained in C. atrox was deleted from the neurotoxic Mojave rattlesnake (C. scutulatus; ∼4 mya). The rapid evolution of PLA2 gene number appears to be due to transposon invasion that provided a template for non-allelic homologous recombination. •Contrary to assumptions, the most recent common rattlesnake ancestor was neurotoxic•Gene number in the phospholipase A2 complex is evolutionarily dynamic•Neurotoxin and myotoxin genes were lost independently in different lineages•Venom diversity is due to both gene duplication and gene loss Dowell et al. show that a phospholipase A2 gene complex encoding a family of venom toxins expanded early in rattlesnake evolution and that individual neurotoxin or myotoxin genes were independently deleted recently in different species lineages. These losses presumably reflect ecological shifts in prey choice that made certain toxins dispensable.