Details

Autor(en) / Beteiligte

• Iraporda, Carolina
• Romanin, David E
• Bengoa, Ana A
• Errea, Agustina J
• Cayet, Delphine
• Foligné, Benoit
• Sirard, Jean-Claude
• Garrote, Graciela L
• Abraham, Analía G
• Rumbo, Martín

Titel

Local Treatment with Lactate Prevents Intestinal Inflammation in the TNBS-Induced Colitis Model

Ist Teil von

• Frontiers in immunology, 2016-12, Vol.7, p.651-651

Ort / Verlag

Switzerland: Frontiers Research Foundation

Erscheinungsjahr

2016

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Lactate has long been considered as a metabolic by-product of cells. Recently, this view has been changed by the observation that lactate can act as a signaling molecule and regulates critical functions of the immune system. We previously identified lactate as the component responsible for the modulation of innate immune epithelial response of fermented milk supernatants . We have also shown that lactate downregulates proinflammatory responses of macrophages and dendritic cells. So far, effects of lactate on intestinal inflammation have not been reported. We evaluated the effect of intrarectal administration of lactate in a murine model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). The increase in lactate concentration in colon promoted protective effects against TNBS-induced colitis preventing histopathological damage, as well as bacterial translocation and rise of IL-6 levels in serum. Using intestinal epithelial reporter cells, we found that flagellin treatment induced reporter gene expression, which was abrogated by lactate treatment as well as by glycolysis inhibitors. Furthermore, lactate treatment modulated glucose uptake, indicating that high levels of extracellular lactate can impair metabolic reprograming induced by proinflammatory activation. These results suggest that lactate could be a potential beneficial microbiota metabolite and may constitute an overlooked effector with modulatory properties.