Details

Autor(en) / Beteiligte

• Harrison, Rane A
• Engen, John R

Titel

Conformational insight into multi-protein signaling assemblies by hydrogen–deuterium exchange mass spectrometry

Ist Teil von

• Current opinion in structural biology, 2016-12, Vol.41, p.187-193

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• [Display omitted] •Hydrogen–deuterium exchange mass spectrometry (HDX MS) is now more routine.•Large signaling proteins can be studied with HDX MS, from kinases to GPCRs.•Membrane mimetics and HDX MS probe signaling complexes at the membrane.•HDX MS with membranes elucidates the role of membrane in conformation.•HDX MS can be leveraged to facilitate X-ray crystallography of large complexes. Hydrogen–deuterium exchange (HDX) mass spectrometry (MS) can provide information about proteins that can be challenging to obtain by other means. Structure/function relationships, binding interactions, and the effects of modification have all been measured with HDX MS for a diverse and growing array of signaling proteins and multiprotein signaling complexes. As a result of hardware and software improvements, receptors and complexes involved in cellular signaling—including those associated with membranes—can now be studied. The growing body of HDX MS studies of signaling complexes at membranes is particularly exciting. Recent examples are presented to illustrate what can be learned about signaling proteins with this technique.