Details

Autor(en) / Beteiligte

• Fagegaltier, Delphine
• Falciatori, Ilaria
• Czech, Benjamin
• Castel, Stephane
• Perrimon, Norbert
• Simcox, Amanda
• Hannon, Gregory J

Titel

Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway

Ist Teil von

• Genes & development, 2016-07, Vol.30 (14), p.1623-1635

Ort / Verlag

United States: Cold Spring Harbor Laboratory Press

Erscheinungsjahr

2016

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Germline genes often become re-expressed in soma-derived human cancers as "cancer/testis antigens" (CTAs), and piRNA (PIWI-interacting RNA) pathway proteins are found among CTAs. However, whether and how the piRNA pathway contributes to oncogenesis in human neoplasms remain poorly understood. We found that oncogenic Ras combined with loss of the Hippo tumor suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from annotated generative loci, which are normally restricted to either the germline or the somatic follicle cells. Negating the pathway leads to increases in the expression of a wide variety of transposons and also altered expression of some protein-coding genes. This correlates with a reduction in the proliferation of the transformed cells in culture, suggesting that, at least in this context, the piRNA pathway may play a functional role in cancer.