Details

Autor(en) / Beteiligte

• Arici, Vittorio
• Perotti, Cesare
• Fabrizio, Calliada
• Del Fante, Claudia
• Ragni, Franco
• Alessandrino, Francesco
• Viarengo, Gianluca
• Pagani, Michele
• Moia, Alessia
• Tinelli, Carmine
• Bozzani, Antonio

Titel

Autologous immuno magnetically selected CD133+ stem cells in the treatment of no-option critical limb ischemia: clinical and contrast enhanced ultrasound assessed results in eight patients

Ist Teil von

• Journal of translational medicine, 2015-11, Vol.13 (342), p.342, Article 342

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2015

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Demonstrate the safety and effectiveness of highly purified CD133+ autologous stem cells in critical limb ischemia (CLI). Prospective single-center not randomized. Clinicaltrials.gov identifier: NCT01595776 METHODS: Eight patients with a history of stable CLI were enrolled in a period of 2 years. After bone marrow stimulation and single leukapheresis collection, CD133+ immunomagnetic cell selection was performed. CD133+ cells in buffer phosphate suspension was administered intramuscularly. Muscular and arterial contrast enhanced ultra sound (CEUS), lesion evolution and pain management were assessed preoperatively and 3, 6 and 12 months after the implant. No patient had early or late complications related to the procedure. Two patients (25 %) didn't get any relief from the treatment and underwent major amputation. Six patients (75 %) had a complete healing of the wounds, rest pain cessation and walking recovery. An increase in CEUS values was shown in all eight patients at 6 months and in the six clinical healed patients at 12 months and had statistical relevance. Highly purified autologous CD133+ cells can stimulate neo-angiogenesis, as based on clinical and CEUS data.