Details

Autor(en) / Beteiligte

• Hu, Linping
• Cheng, Hui
• Gao, Yingdai
• Shi, Ming
• Liu, Yanfeng
• Hu, Zheng
• Xu, Jing
• Qiu, Lugui
• Yuan, Weiping
• Leung, Anskar Yu-Hung
• Yang, Yong-Guang
• Cheng, Tao

Titel

Antioxidant N-acetyl-l-cysteine increases engraftment of human hematopoietic stem cells in immune-deficient mice

Ist Teil von

• Blood, 2014-11, Vol.124 (20), p.e45-e48

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Immunocompromised mice, such as the nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice, have been widely used to examine the self-renewal and differentiation potential of human hematopoietic stem cells (HSCs) in vivo. However, the efficiency of human HSC engraftment remains very low. Here, we report that NOD/SCID mice had higher levels of reactive oxygen species (ROS) in their bone marrow (BM) than other commonly used mouse strains (C57BL/6 and BALB/C). Treatment with the antioxidant N-acetyl-l-cysteine (NAC) decreased ROS levels in the BM of NOD/SCID mice. Furthermore, the NAC-treated mice displayed a significant increase in human HSC engraftment and multilineage hematopoietic differentiation in the mice. In comparison with the control mice, NAC-treated recipients displayed a 10.8-fold increase in hematopoietic engraftment in the injected tibiae. A beneficial effect of NAC for human hematopoietic engraftment was also observed in an additional immunodeficient mouse strain, namely NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NOD/SCID/γc−/− or NSG). Thus, this study uncovers a previously unappreciated negative effect of ROS on human stem cell engraftment in immunodeficient mice. •NAC increases engraftment of human hematopoietic stem cells in immunodeficient mice.