Details
- Autor(en) / Beteiligte
- Titel
- The mitochondrial contact site complex, a determinant of mitochondrial architecture
- Ist Teil von
- The EMBO journal, 2011-11, Vol.30 (21), p.4356-4370
- Ort / Verlag
- Chichester, UK: John Wiley & Sons, Ltd
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- Mitochondria are organelles with a complex architecture. They are bounded by an envelope consisting of the outer membrane and the inner boundary membrane (IBM). Narrow crista junctions (CJs) link the IBM to the cristae. OMs and IBMs are firmly connected by contact sites (CS). The molecular nature of the CS remained unknown. Using quantitative high‐resolution mass spectrometry we identified a novel complex, the mitochondrial contact site (MICOS) complex, formed by a set of mitochondrial membrane proteins that is essential for the formation of CS. MICOS is preferentially located at the CJs. Upon loss of one of the MICOS subunits, CJs disappear completely or are impaired, showing that CJs require the presence of CS to form a superstructure that links the IBM to the cristae. Loss of MICOS subunits results in loss of respiratory competence and altered inheritance of mitochondrial DNA. The outer and inner mitochondrial membranes are physically linked. Quantitative high resolution mass spectrometry now identifies the molecular nature of the Mitochondrial Contact Site complex (MICOS). MICOS is required for crista junctions formation, respiration and mitochondrial DNA inheritance.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0261-4189eISSN: 1460-2075DOI: 10.1038/emboj.2011.379Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3230385
- Format
- –
- Schlagworte
- Binding Sites - physiology, Cellular biology, contact site proteins, crista junction, DNA, Mitochondrial - metabolism, Intracellular Membranes - metabolism, Membrane Proteins - genetics, Membrane Proteins - metabolism, Membranes, MICOS, Microscopy, Electron, Mitochondria, Mitochondria - chemistry, Mitochondria - metabolism, Mitochondria - ultrastructure, mitochondrial membrane proteome, Mitochondrial Proteins - chemistry, Mitochondrial Proteins - metabolism, Mitochondrial Proteins - physiology, Models, Biological, molecular architecture of mitochondria, Molecular biology, Multiprotein Complexes - chemistry, Multiprotein Complexes - metabolism, Multiprotein Complexes - physiology, Organisms, Genetically Modified, Protein Binding - genetics, Protein Binding - physiology, Protein Transport, Proteomics, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae - ultrastructure