Details

Autor(en) / Beteiligte

• Yan, Zier
• Wang, Huanchen
• Cai, Jiwen
• Ke, Hengming

Titel

Refolding and kinetic characterization of the phosphodiesterase-8A catalytic domain

Ist Teil von

• Protein expression and purification, 2009-03, Vol.64 (1), p.82-88

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Cyclic nucleotide phosphodiesterase-8 (PDE8) hydrolyzes the second messenger cAMP and is involved in many biological processes such as testosterone production. Although the bacterial and mammalian expression systems have been extensively tried, production of large quantity of soluble and active PDE8 remains to be a major hurdle for pharmacological and structural studies. Reported here is a detailed protocol of refolding and purification of large quantity of the PDE8A1 catalytic domain (residues 480–820) and kinetic characterization of the refolded protein. This protocol yielded about 8 mg of the PDE8A catalytic domain from 2 l Escherichia coli culture, which has at least 40-fold higher activity than those reported in literature. The PDE8A1 catalytic domain has k cat of 4.0 s −1 for Mn 2+ and 2.9 s −1 for Mg 2+, and the K M values of 1–1.8 μM. In addition, the PDE8A1 (205–820) fragment that contains both PAS and catalytic domains was expressed in E. coli and refolded. This PDE8A1 (205–820) fragment has k cat of 1.1 s −1 and K M of 0.28 μM, but aggregated at high concentration. The K M of PDE8A1 (205–820) is 2- to 7-fold higher than the K M values of 40–150 nM for the full-length PDE8s in literature, but about 6-fold lower than that of the catalytic domain. Thus, the K M difference likely implies an allosteric regulation of the PDE8A activity by its PAS domain.