The Journal of cell biology, 2005-07, Vol.170 (2), p.249-260
2005
Details
- Autor(en) / Beteiligte
- Titel
- SNAREs can promote complete fusion and hemifusion as alternative outcomes
- Ist Teil von
- The Journal of cell biology, 2005-07, Vol.170 (2), p.249-260
- Ort / Verlag
- United States: Rockefeller University Press
- Erscheinungsjahr
- 2005
- Link zum Volltext
- Quelle
- Electronic Journals Library - Freely accessible e-journals
- Beschreibungen/Notizen
- Using a cell fusion assay, we show here that in addition to complete fusion SNAREs also promote hemifusion as an alternative outcome. Approximately 65% of events resulted in full fusion, and the remaining 35% in hemifusion; of those, approximately two thirds were permanent and approximately one third were reversible. We predict that this relatively close balance among outcomes could be tipped by binding of regulatory proteins to the SNAREs, allowing for dynamic physiological regulation between full fusion and reversible kiss-and-run-like events.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9525eISSN: 1540-8140DOI: 10.1083/jcb.200501093Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2171417
- Format
- –
- Schlagworte
- 3T3 Cells, Animals, Cell fusion, Cell lines, Cell Membrane - physiology, Cell membranes, Cellular biology, CHO Cells, COS cells, Cricetinae, Cricetulus, Fusion, Lipids, Membrane Fusion - genetics, Membrane Fusion - physiology, Mice, P branes, Plasmids, Protein Conformation, Recombinant Proteins - genetics, Recombinant Proteins - metabolism, SNARE Proteins, T lymphocytes, Vesicular Transport Proteins - chemistry, Vesicular Transport Proteins - genetics, Vesicular Transport Proteins - metabolism