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Protein science, 1997-10, Vol.6 (10), p.2043-2058
1997

Details

Autor(en) / Beteiligte
Titel
Sequence analysis of the AAA protein family
Ist Teil von
  • Protein science, 1997-10, Vol.6 (10), p.2043-2058
Ort / Verlag
Bristol: Cold Spring Harbor Laboratory Press
Erscheinungsjahr
1997
Link zum Volltext
Quelle
EZB-FREE-00999 freely available EZB journals
Beschreibungen/Notizen
  • The AAA protein family, a recently recognized group of Walker‐type ATPases, has been subjected to an extensive sequence analysis. Multiple sequence alignments revealed the existence of a region of sequence similarity, the so‐called AAA cassette. The borders of this cassette were localized and within it, three boxes of a high degree of conservation were identified. Two of these boxes could be assigned to substantial parts of the ATP binding site (namely, to Walker motifs A and B); the third may be a portion of the catalytic center. Phylogenetic trees were calculated to obtain insights into the evolutionary history of the family. Subfamilies with varying degrees of intra‐relatedness could be discriminated; these relationships are also supported by analysis of sequences outside the canonical AAA boxes: within the cassette are regions that are strongly conserved within each subfamily, whereas little or even no similarity between different subfamilies can be observed. These regions are well suited to define fingerprints for subfamilies. A secondary structure prediction utilizing all available sequence information was performed and the result was fitted to the general 3D structure of a Walker A/GTPase. The agreement was unexpectedly high and strongly supports the conclusion that the AAA family belongs to the Walker superfamily of A/GTPases.
Sprache
Englisch
Identifikatoren
ISSN: 0961-8368
eISSN: 1469-896X
DOI: 10.1002/pro.5560061001
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2143574

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