Details

Autor(en) / Beteiligte

• Kleerebezem, Michiel
• Boekhorst, Jos
• van Kranenburg, Richard
• Molenaar, Douwe
• Kuipers, Oscar P.
• Leer, Rob
• Tarchini, Renato
• Peters, Sander A.
• Sandbrink, Hans M.
• Mark W. E. J. Fiers
• Stiekema, Willem
• René M. Klein Lankhorst
• Bron, Peter A.
• Hoffer, Sally M.
• Masja N. Nierop Groot
• Kerkhoven, Robert
• de Vries, Maaike
• Ursing, Björn
• de Vos, Willem M.
• Siezen, Roland J.

Titel

Complete Genome Sequence of Lactobacillus plantarum WCFS1

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2003-02, Vol.100 (4), p.1990-1995

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2003

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes. Putative biological functions could be assigned to 2,120 (70%) of the predicted proteins. Consistent with the classification of L. plantarum as a facultative heterofermentative lactic acid bacterium, the genome encodes all enzymes required for the glycolysis and phosphoketolase pathways, all of which appear to belong to the class of potentially highly expressed genes in this organism, as was evident from the codon-adaptation index of individual genes. Moreover, L. plantarum encodes a large pyruvate-dissipating potential, leading to various end-products of fermentation. L. plantarum is a species that is encountered in many different environmental niches, and this flexible and adaptive behavior is reflected by the relatively large number of regulatory and transport functions, including 25 complete PTS sugar transport systems. Moreover, the chromosome encodes >200 extracellular proteins, many of which are predicted to be bound to the cell envelope. A large proportion of the genes encoding sugar transport and utilization, as well as genes encoding extracellular functions, appear to be clustered in a 600-kb region near the origin of replication. Many of these genes display deviation of nucleotide composition, consistent with a foreign origin. These findings suggest that these genes, which provide an important part of the interaction of L. plantarum with its environment, form a lifestyle adaptation region in the chromosome.