Details

Autor(en) / Beteiligte

• Mariottini, Davide
• Bracaglia, Sara
• Barbero, Luca
• Fuchs, Sebastian W.
• Saal, Christoph
• Moniot, Sebastien
• Knuehl, Christine
• Baranda, Lorena
• Ranallo, Simona
• Ricci, Francesco

Titel

Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands

Ist Teil von

• ACS sensors, 2023-11, Vol.8 (11), p.4014-4019

Ort / Verlag

American Chemical Society

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We report here the development of two different sensing strategies based on the use of antigen-conjugated nucleic acid strands for the detection of a bispecific antibody against the tumor-related proteins Mucin1 and epidermal growth factor receptor. Both approaches work well in serum samples (nanomolar sensitivity), show high specificity against the two monospecific antibodies, and are rapid. The results presented here demonstrate the versatility of DNA-based platforms for the detection of bispecific antibodies and could represent a versatile alternative to other more reagent-intensive and time-consuming analytical approaches.