Nature methods, 2016-06, Vol.13 (6), p.497-500
2016
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Details
- Autor(en) / Beteiligte
- Titel
- An unbiased metric of antiproliferative drug effect in vitro
- Ist Teil von
- Nature methods, 2016-06, Vol.13 (6), p.497-500
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and drug discovery. Using theoretical modeling and experimentation, we show that current metrics of antiproliferative small molecule effect suffer from time-dependent bias, leading to inaccurate assessments of parameters such as drug potency and efficacy. We propose the drug-induced proliferation (DIP) rate, the slope of the line on a plot of cell population doublings versus time, as an alternative, time-independent metric.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/nmeth.3852Titel-ID: cdi_pubmed_primary_27135974
- Format
- –
- Schlagworte
- Cell Line, Tumor, Cell Proliferation - drug effects, Computer Simulation, Dose-Response Relationship, Drug, Drug Discovery - methods, Humans, Microscopy, Fluorescence, Models, Theoretical, Molecular Biology - methods, Sensitivity and Specificity, Small Molecule Libraries - chemistry, Small Molecule Libraries - pharmacology, Time Factors