Details

Autor(en) / Beteiligte

• Halbmayr-Jech, Elisabeth
• Rogers, Adrian
• Don, Clyde
• Prinster, Michael

Titel

Gluten in Rice Flour and Baked Rice Products by G12 Sandwich ELISA: First Action 2014.03

Ist Teil von

• Journal of AOAC International, 2015-01, Vol.98 (1), p.103-111

Ort / Verlag

England

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• The Protein and Enzymes Technical Committee of American Association of Cereal Chemists initiated a collaborative study to confirm whether the G12 antibody-based sandwich ELISA test kit is able to detect gluten in the lower mg/kg (ppm) level. Twenty laboratories investigated 24 heat-treated and non-heat-treated blind-coded samples with incurred gluten levels up to 100 mg/kg. The method has been validated for testing foods to conform to the defined Codex thresholds for gluten in gluten-free products at less than 20 mg gluten/kg. The collaborative study showed that low levels of gluten could be detected by G12 Sandwich ELISA with reproducibility RSDR of 32% and repeatability RSDr of 16%. Incurred samples showed a recovery between 62 and 135%. It is recommended that the method be accepted by AOAC as Official First Action.