Details

Autor(en) / Beteiligte

• Aguilar-Arnal, Lorena
• Hakim, Ofir
• Patel, Vishal R
• Baldi, Pierre
• Hager, Gordon L
• Sassone-Corsi, Paolo

Titel

Cycles in spatial and temporal chromosomal organization driven by the circadian clock

Ist Teil von

• Nature structural & molecular biology, 2013-10, Vol.20 (10), p.1206

Ort / Verlag

United States

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Dynamic transitions in the epigenome have been associated with regulated patterns of nuclear organization. The accumulating evidence that chromatin remodeling is implicated in circadian function prompted us to explore whether the clock may control nuclear architecture. We applied the chromosome conformation capture on chip technology in mouse embryonic fibroblasts (MEFs) to demonstrate the presence of circadian long-range interactions using the clock-controlled Dbp gene as bait. The circadian genomic interactions with Dbp were highly specific and were absent in MEFs whose clock was disrupted by ablation of the Bmal1 gene (also called Arntl). We establish that the Dbp circadian interactome contains a wide variety of genes and clock-related DNA elements. These findings reveal a previously unappreciated circadian and clock-dependent shaping of the nuclear landscape.