Details

Autor(en) / Beteiligte

• Bakker, Remko A
• Jongejan, Aldo
• Sansuk, Kamonchanok
• Hacksell, Uli
• Timmerman, Henk
• Brann, Mark R
• Weiner, Dave M
• Pardo, Leonardo
• Leurs, Rob

Titel

Constitutively Active Mutants of the Histamine H1 Receptor Suggest a Conserved Hydrophobic Asparagine-Cage That Constrains the Activation of Class A G Protein-Coupled Receptors

Ist Teil von

• Molecular pharmacology, 2008-01, Vol.73 (1), p.94

Ort / Verlag

United States: American Society for Pharmacology and Experimental Therapeutics

Erscheinungsjahr

2008

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The aim of this study was to create and characterize constitutively active mutant (CAM) histamine H 1 receptors (H 1 R) using random mutagenesis methods to further investigate the activation process of the rhodopsin-like family of G protein-coupled receptors (GPCRs). This approach identified position 6.40 in TM 6 as a “hot spot” because mutation of Ile6.40 420 either to Glu, Gly, Ala, Arg, Lys, or Ser resulted in highly active CAM H 1 Rs, for which almost no histamine-induced receptor activation response could be detected. The highly conserved hydrophobic amino acid at position 6.40 defines, in a computational model of the H 1 R, the asparagine cage motif that restrains the side chain of Asn7.49 of the NPxxY motif toward transmembrane domain (TM 6) in the inactive state of the receptor. Mutation of the asparagine cage into Ala or Gly, removing the interfering bulky constraints, increases the constitutive activity of the receptor. The fact that the Ile6.40 420 Arg/Lys/Glu mutant receptors are highly active CAM H 1 Rs leads us to suggest that a positively charged residue, presumably the highly conserved Arg3.50 from the DRY motif, interacts in a direct or an indirect (through other side chains or/and internal water molecules) manner with the acidic Asp2.50··Asn7.49 pair for receptor activation.