Nucleic acids research, 2003-02, Vol.31 (4), p.1292
2003
Details
- Autor(en) / Beteiligte
- Titel
- Characterization of the human beta-globin downstream promoter region
- Ist Teil von
- Nucleic acids research, 2003-02, Vol.31 (4), p.1292
- Ort / Verlag
- England
- Erscheinungsjahr
- 2003
- Link zum Volltext
- Beschreibungen/Notizen
- The human beta-globin gene is abundantly expressed specifically in adult erythroid cells. Stage-specific transcription is regulated principally by promoter proximal cis-regulatory elements. The basal promoter contains a non-canonical TATA-like motif as well as an initiator element. These two elements have been shown to interact with the TFII-D complex. Here we show that in addition to the TATA and initiator elements, conserved E-box motifs are located in the beta-globin downstream promoter. One of the E-box motifs overlaps the initiator and this composite element interacts with USF1 and TFII-I in vitro. Another E-box, located 60 bp 3' to the transcription initiation site, interacts with USF1 and USF2. Mutations of either the initiator or the downstream E-box impair transcription of the beta-globin gene in vitro. Mutations of a putative NF-E2-binding site in the downstream promoter region do not affect transcription in vitro. USF1, USF2, TFII-I and p45 can be crosslinked to a beta-globin promoter fragment in MEL cells in vivo, whereas only TFII-I and USF2 crosslink to the beta-globin gene in K562 cells. The summary data demonstrate that in addition to the well-characterized interactions of the TFII-D complex with the basal promoter, E-box motifs contribute to the efficient formation of transcription complexes on the adult beta-globin gene.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1362-4962DOI: 10.1093/nar/gkg209Titel-ID: cdi_pubmed_primary_12582249
- Format
- –
- Schlagworte
- 3T3 Cells, Animals, Base Sequence, Binding Sites - genetics, DNA-Binding Proteins - metabolism, Electrophoretic Mobility Shift Assay, Erythroid-Specific DNA-Binding Factors, Globins - genetics, Globins - metabolism, Humans, K562 Cells, Mice, Models, Biological, Molecular Sequence Data, Mutation, NF-E2 Transcription Factor, NF-E2 Transcription Factor, p45 Subunit, Promoter Regions, Genetic - genetics, Protein Binding, Sequence Homology, Nucleic Acid, Transcription Factors - metabolism, Transcription Factors, TFII - metabolism, Tumor Cells, Cultured, Upstream Stimulatory Factors