Details

Autor(en) / Beteiligte

• Parkes, G. C.
• Rayment, N. B.
• Hudspith, B. N.
• Petrovska, L.
• Lomer, M. C.
• Brostoff, J.
• Whelan, K.
• Sanderson, J. D.

Titel

Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome

Ist Teil von

• Neurogastroenterology and motility, 2012-01, Vol.24 (1), p.31-39

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2012

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Background  There is increasing evidence to support a role for the gastrointestinal microbiota in the etiology of irritable bowel syndrome (IBS). Given the evidence of an inflammatory component to IBS, the mucosa‐associated microbiota potentially play a key role in its pathogenesis. The objectives were to compare the mucosa‐associated microbiota between patients with diarrhea predominant IBS (IBS‐D), constipation predominant IBS (IBS‐C) and controls using fluorescent in situ hybridization and to correlate specific bacteria groups with individual IBS symptoms. Methods  Forty‐seven patients with IBS (27 IBS‐D and 20 IBS‐C) and 26 healthy controls were recruited to the study. Snap‐frozen rectal biopsies were taken at colonoscopy and bacterial quantification performed by hybridizing frozen sections with bacterial‐group specific oligonucleotide probes. Key Results  Patients with IBS had significantly greater numbers of total mucosa‐associated bacteria per mm of rectal epithelium than controls [median 218 (IQR – 209) vs 128 (121) P = 0.007], and this was chiefly comprised of bacteroides IBS [69 (67) vs 14 (41) P = 0.001] and Eubacterium rectale–Clostridium coccoides [52 (58) vs 25 (35) P = 0.03]. Analysis of IBS sub‐groups demonstrated that bifidobacteria were lower in the IBS‐D group than in the IBS‐C group and controls [24 (32) vs 54 (88) vs 32 (35) P = 0.011]. Finally, amongst patients with IBS, the maximum number of stools per day negatively correlated with the number of mucosa‐associated bifidobacteria (P < 0.001) and lactobacilli (P = 0.002). Conclusions & Inferences  The mucosa‐associated microbiota in patients with IBS is significantly different from healthy controls with increases in bacteroides and clostridia and a reduction in bifidobacteria in patients with IBS‐D.