Details

Autor(en) / Beteiligte

• Kanimozhi, Sivarajan
• Basheer, Chanbasha
• Narasimhan, Kothandaraman
• Liu, Lin
• Koh, Stephen
• Xue, Feng
• Choolani, Mahesh
• Lee, Hian Kee

Titel

Application of porous membrane protected micro-solid-phase-extraction combined with gas chromatography–mass spectrometry for the determination of estrogens in ovarian cyst fluid samples

Ist Teil von

• Analytica chimica acta, 2011-02, Vol.687 (1), p.56-60

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2011

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• A cost effective and environmentally friendly extraction technique using porous membrane protected micro-solid phase extraction (μ-SPE) is described for the extraction of estrogens in cyst fluid samples obtained from cancer patients. A sorbent (ethylsilane (C2) modified silica) (20 mg) was packed in a porous polypropylene envelope (2 cm × 1.5 cm) whose edges were heat sealed to secure the contents. The μ-SPE device was conditioned with acetone and placed in a stirred (1:5) diluted cyst fluid sample solution (10 mL) to extract estrogens for 60 min. After extraction, the analytes were desorbed and simultaneously derivatized with a 5:1 mixture of acetone and N, O-bis(trimethylsilyl)-trifluoroacetamide. The extract (2 μL) was analyzed by gas chromatography–mass spectrometry. Various extraction, desorption and derivatization conditions were optimized for μ-SPE. With this simple technique, low limits of detection of between 9 and 22 ng L −1 and linear range from the detection limits up to 50 μg L −1 were achieved. The optimized method was used to extract estrogens from cyst fluid samples obtained from patients with malignant and benign ovarian tumors.