Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
ATP induces Ca2+ release from IP3-sensitive Ca2+ stores exclusively in large DRG neurones
Ist Teil von
Neuroreport, 1997-05, Vol.8 (7), p.1555-1559
Ort / Verlag
Hagerstown, MD: Lippincott-Raven Publishers
Erscheinungsjahr
1997
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
PURINORECEPTOR-MEDIATED intracellular Ca release was studied in freshly isolated adult mouse dorsal root ganglia (DRG) neurones. The cytoplasmic Ca concentration ([Ca]i) was measured using indo-1-based microfluorimetry. The application of 100 μM ATP in Ca-free solution triggered an increase in [Ca]i in 93% of large DRG neurones but in no small ones. The ATP-induced [Ca]i transients in large neurones were inhibited by cells incubation with thapsigargin or by intracellular dialysis with heparin-containing solution. The ATP-triggered increase in [Ca]i was not mimicked by adenosine and was blocked by suramin, suggesting the involvement of metabotropic (P2Y) purinoreceptors. We conclude that large (proprioceptive) DRG neurones express P2Y purinoreceptors linked to the inositol 1,4,5-triphosphate-Ca intracellular signal transduction cascade, whereas small (nociceptive) DRG neurones are devoid of such a mechanism.