Details

Autor(en) / Beteiligte

• Puhl, Herbert
• Waeg, Georg
• Esterbauer, Hermann

Titel

[46] Methods to determine oxidation of low-density lipoproteins

Ist Teil von

• Methods in Enzymology, 1994, Vol.233, p.425-441

Ort / Verlag

United States: Elsevier Science & Technology

Erscheinungsjahr

1994

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Low-density lipoprotein (LDL) is an important mediator in the pathogenesis of atherosclerosis. This chapter introduces methods to determine oxidation of low-density lipoproteins. Evaluation of lipoprotein oxidation in vivo, however, is difficult, and most of the investigations deal with in vitro oxidized LDL. During oxidation of LDL by cells or in cell-free systems the composition of the lipoprotein particle progressively changes with time; thus, continuous kinetic measurements or multiple analyses at different time intervals are necessary to assess the kinetics of the oxidation process. Oxidation of LDL in vitro is accompanied by characteristic changes of chemical, physicochemical, and biological properties, and a variety of methods may therefore be used for determining the extent and/or rate of oxidation of LDL. They include measurement of the increase of thiobarbituric acid-reactive substances (TBARS), total lipid hydroperoxides, defined lipid hydroperoxides, hydroxy and hydroperoxy fatty acids, conjugated dienes, oxysterols, lysophosphatides, aldehydes, and fluorescent chromophores, and measurements of the disappearance of endogenous antioxidants and polyunsaturated fatty acids, and oxygen uptake.