Details

Autor(en) / Beteiligte

• Moraes, R.M.A. de
• Soares, T.C.B
• Colombo, L.R
• Salla, M.F.S
• Barros, J.G. de A
• Piovesan, N.D
• Barros, E.G. de
• Moreira, M.A

Titel

Assisted Selection by Specific DNA Markers for Genetic Elimination of the Kunitz Trypsin Inhibitor and Lectin in Soybean Seeds

Ist Teil von

• Euphytica, 2006-05, Vol.149 (1-2), p.221-226

Ort / Verlag

Dordrecht: Springer

Erscheinungsjahr

2006

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The antinutritional factors found in soybean, lectin (SBL) and Kunitz trypsin inhibitor (SKTI), are usually inactivated by heat treatment. However, residual activity of these factors can be found in several types of soy-derived products. Heat treatment does not completely eliminate these factors, and in addition it may considerably decrease protein solubility. The genetic elimination of these antinutritional factors could be an alternative to the heat treatment. This study aimed to develop soybean lines devoid of SKTI and SBL in their seeds. The population under study was obtained by crossing the normal cv. Monarca with a soybean line lacking SKTI and SBL. Specific DNA primers were designed for the identification of the recessive alleles that condition the absence of SKTI and SBL. F sub(2) seeds presenting the DNA markers that identify the recessive alleles were selected and the corresponding F sub(2) plants were backcrossed with the recurrent parent ('Monarca'), producing the BC sub(1)F sub(1) generation. The F sub(2) generation was analyzed by SDS-PAGE in order to confirm the genotypes of the F sub(2) selected. The segregation tests confirmed that these traits are governed by two genes that segregate independently.