Science (American Association for the Advancement of Science), 2001-02, Vol.291 (5506), p.1047-1051
2001
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Details
- Autor(en) / Beteiligte
- Titel
- Role of the ENTH Domain in Phosphatidylinositol-4,5-Bisphosphate Binding and Endocytosis
- Ist Teil von
- Science (American Association for the Advancement of Science), 2001-02, Vol.291 (5506), p.1047-1051
- Ort / Verlag
- Washington, DC: American Society for the Advancement of Science
- Erscheinungsjahr
- 2001
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Endocytic proteins such as epsin, AP180, and Hip1R (Sla2p) share a conserved modular region termed the epsin NH2-terminal homology (ENTH) domain, which plays a crucial role in clathrin-mediated endocytosis through an unknown target. Here, we demonstrate a strong affinity of the ENTH domain for phosphatidylinositol-4,5-bisphosphate [Ptdlns(4,5)P2]. With nuclear magnetic resonance analysis of the epsin ENTH domain, we determined that a cleft formed with positively charged residues contributed to phosphoinositide binding. Over-expression of a mutant, epsin Lys76→ Ala76, with an ENTH domain defective in phosphoinositide binding, blocked epidermal growth factor internalization in COS-7 cells. Thus, interaction between the ENTH domain and Ptdlns(4,5)P2is essential for endocytosis mediated by clathrin-coated pits.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.291.5506.1047Titel-ID: cdi_proquest_miscellaneous_743418209
- Format
- –
- Schlagworte
- Adaptor Proteins, Vesicular Transport, Amino Acid Motifs, Amino acid structure-activity relationships, Amino Acid Substitution, Amino acids, Animals, Biochemistry, Biological and medical sciences, Bulk amino acids, Carrier Proteins - chemistry, Carrier Proteins - metabolism, Cell membranes, Cell physiology, Cercopithecus aethiops, Chemical equilibrium, Clathrin - metabolism, Coated Pits, Cell-Membrane - metabolism, COS Cells, DNA-Binding Proteins - metabolism, Endocytosis, Epidermal Growth Factor - metabolism, Fundamental and applied biological sciences. Psychology, Genes, Inositol Phosphates - metabolism, Internalization, Liposomes, Liposomes - metabolism, Models, Molecular, Molecular and cellular biology, Mutation, Neuropeptides - chemistry, Neuropeptides - metabolism, Nuclear Magnetic Resonance, Biomolecular, Phosphatidylinositol, Phosphatidylinositol 4,5-Diphosphate - metabolism, Phosphatidylinositols, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Proteins, Recombinant Fusion Proteins - chemistry, Recombinant Fusion Proteins - metabolism, Structure-activity relationships, Transcription Factors - metabolism, Vesicular Transport Proteins, Yeasts, Zinc Fingers