Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Rapid cloning of specific DNA fragments of Streptococcus pneumoniae by vector integration into the chromosome followed by endonucleolytic excision
Ist Teil von
Gene, 1981-01, Vol.15 (2), p.289-293
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
1981
Link zum Volltext
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
A method for the rapid cloning of specific
Streptococcus pneumoniae DNA fragments depends on the integration by homologous recombination into the bacterial chromosome of a plasmid which carries an insert of
S. pneumoniae DNA, but which cannot be autonomously maintained in
S. pneumoniae. Selection for plasmid integration employs aminopterin or erythromycin resistance. Host sequences adjacent to the site of insertion are easily cloned by enzymatic excision and recirculation of the plasmid, followed by propagation in
Escherichia coli. This is particularly useful for repeated cloning of a given fragment that carries various mutations