Biochemical and biophysical research communications, 2010-01, Vol.391 (1), p.49-55
2010
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- Autor(en) / Beteiligte
- Titel
- Prolonged tyrosine kinase activation of insulin receptor by pY27-caveolin-2
- Ist Teil von
- Biochemical and biophysical research communications, 2010-01, Vol.391 (1), p.49-55
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Caveolin-2 regulation of insulin receptor (IR) tyrosine kinase activity was investigated. An insulin time course revealed that rapidly induced tyrosine phosphorylation of IR was steadily maintained over a 180min time period. In parallel, insulin-exerted IR interaction with caveolin-2 was detected as early as 5min throughout until 180min. Down-regulation of caveolin-2 by caveolin-2 siRNA arrested specifically a long term activation of IR. The attenuation of IR activation resulted in retardation of rapamycin-sensitive pS727-STAT3 activation. As caveolin-2 tyrosine mutants were examined, Y27A-caveolin-2 explicitly impeded the long term IR activation by insulin, enhanced tyrosine dephosphorylation of IR, impaired tyrosine phosphorylation of IRS-1, and exerted the interaction between activated IR and SOCS-3. Together, we propose that pY27-caveolin-2 prolongs IR activation by its interaction with IR, thereby preventing IR interaction with SOCS-3.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-291XeISSN: 1090-2104DOI: 10.1016/j.bbrc.2009.10.159Titel-ID: cdi_proquest_miscellaneous_733705807
- Format
- –
- Schlagworte
- Animals, Caveolin 2 - genetics, Caveolin 2 - metabolism, Cell Line, Enzyme Activation, Humans, Insulin receptor, Insulin Receptor Substrate Proteins - metabolism, IRS-1, Mice, Mutation, Phosphorylation, pS727-STAT3, PTPs, pY27-caveolin-2, Rapamycin, Rats, Receptor, Insulin - biosynthesis, Receptor, Insulin - genetics, RNA, Small Interfering - genetics, SOCS-3, STAT3 Transcription Factor - metabolism, Suppressor of Cytokine Signaling Proteins - metabolism, Tyrosine - genetics, Tyrosine - metabolism