Oncogene, 2002-11, Vol.21 (53), p.8196-8205
2002
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- Autor(en) / Beteiligte
- Titel
- Novel genes regulated by Sonic Hedgehog in pluripotent mesenchymal cells
- Ist Teil von
- Oncogene, 2002-11, Vol.21 (53), p.8196-8205
- Ort / Verlag
- Basingstoke: Nature Publishing
- Erscheinungsjahr
- 2002
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Sonic Hedgehog is a secreted morphogen involved in patterning a wide range of structures in the developing embryo. Disruption of the Hedgehog signalling cascade leads to a number of developmental disorders and plays a key role in the formation of a range of human cancers. The identification of genes regulated by Hedgehog is crucial to understanding how disruption of this pathway leads to neoplastic transformation. We have used a Sonic Hedgehog (Shh) responsive mouse cell line, C3H/10T1/2, to provide a model system for hedgehog target gene discovery. Following activation of cell cultures with Shh, RNA was used to interrogate microarrays to investigate downstream transcriptional consequences of hedgehog stimulation. As a result 11 target genes have been identified, seven of which are induced (Thrombomodulin, GILZ, BF-2, Nr4a1, IGF2, PMP22, LASP1) and four of which are repressed (SFRP-1, SFRP-2, Mip1-gamma, Amh) by Shh. These targets have a diverse range of putative functions and include transcriptional regulators and molecules known to be involved in regulating cell growth or apoptosis. The corroboration of genes previously implicated in hedgehog signalling, along with the finding of novel targets, demonstrates both the validity and power of the C3H/10T1/2 system for Shh target gene discovery.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-9232eISSN: 1476-5594DOI: 10.1038/sj.onc.1205975Titel-ID: cdi_proquest_miscellaneous_72707245
- Format
- –
- Schlagworte
- Animals, Anti-Mullerian Hormone, Apoptosis, Biological and medical sciences, Cancer, Cell activation, Cell Count, Cell physiology, Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes, Cytoskeletal Proteins, Developmental disabilities, DNA microarrays, DNA-Binding Proteins - biosynthesis, DNA-Binding Proteins - genetics, Embryonic development, Forkhead Transcription Factors, Frizzled-related protein 1, Fundamental and applied biological sciences. Psychology, Gene expression, Gene Expression Regulation, Genes, Genetic aspects, Glycoproteins - biosynthesis, Glycoproteins - genetics, Growth Inhibitors - biosynthesis, Growth Inhibitors - genetics, Health aspects, Hedgehog protein, Hedgehog Proteins, Homeodomain Proteins - biosynthesis, Homeodomain Proteins - genetics, Identification and classification, Insulin-like growth factor II, Insulin-Like Growth Factor II - biosynthesis, Insulin-Like Growth Factor II - genetics, LIM Domain Proteins, Membrane Proteins, Mesenchyme, Mesoderm - cytology, Mice, Mice, Inbred C3H, Molecular and cellular biology, Myelin Proteins - biosynthesis, Myelin Proteins - genetics, Neoplasm Proteins, Nerve Tissue Proteins - biosynthesis, Nerve Tissue Proteins - genetics, Nuclear Receptor Subfamily 4, Group A, Member 1, Oligonucleotide Array Sequence Analysis, Pattern formation, Peripheral myelin protein 22, Phosphatase, Physiological aspects, Pluripotency, Pluripotent Stem Cells - metabolism, Protein Biosynthesis, Proteins - genetics, Receptors, Cytoplasmic and Nuclear, Receptors, Steroid, Recombinant Fusion Proteins - physiology, Signal Transduction, Stem cells, Testicular Hormones - biosynthesis, Testicular Hormones - genetics, Thrombomodulin - biosynthesis, Thrombomodulin - genetics, Trans-Activators - genetics, Trans-Activators - physiology, Transcription, Transcription Factors - biosynthesis, Transcription Factors - genetics, Transfection