Experimental neurology, 2004-07, Vol.188 (1), p.187-191
2004
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Details
- Autor(en) / Beteiligte
- Titel
- Wallerian degeneration after crush or chronic constriction injury of rodent sciatic nerve is associated with a depletion of endoneurial interleukin-10 protein
- Ist Teil von
- Experimental neurology, 2004-07, Vol.188 (1), p.187-191
- Ort / Verlag
- Amsterdam: Elsevier Inc
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We used enzyme-linked immunoassay (ELISA), immunohistochemistry (IHC), and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to determine whether interleukin (IL)-10 protein is changed after unilateral crush or chronic constriction injury (CCI) of mouse or rat sciatic nerve and whether IL-10 protein and mRNA are differentially regulated. In the mouse sciatic nerve, IL-10 protein declined rapidly to 10–20% of baseline early after crush or CCI, while the IL10 mRNA was up-regulated with a maximum on Days 1 and 3. In the rat sciatic nerve, IL-10 protein was significantly reduced on Day 3 after CCI, and IL-10 mRNA was up-regulated in both models. These results suggest that changes of the local cytokine network during wallerian degeneration include an early deficiency of the antiinflammatory cytokine IL-10 despite up-regulation at the mRNA level.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-4886eISSN: 1090-2430DOI: 10.1016/j.expneurol.2004.02.011Titel-ID: cdi_proquest_miscellaneous_72012952
- Format
- –
- Schlagworte
- Animals, Axons - metabolism, Axons - pathology, Biological and medical sciences, Chronic Disease, Cytokines, Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases, Disease Models, Animal, Disease Progression, Down-Regulation - genetics, Endoneurium, Epineurium, Female, Inflammation - genetics, Inflammation - immunology, Inflammation - metabolism, Injuries of the nervous system and the skull. Diseases due to physical agents, Interleukin-10 - deficiency, Interleukin-10 - genetics, Interleukin-10 - metabolism, Ligation, Macrophages, Medical sciences, Mice, Mice, Inbred C57BL, Nerve Crush, Neurology, Neuropathic pain, Peripheral nerve injury, Peripheral Nerves - cytology, Peripheral Nerves - metabolism, Peripheral Nervous System Diseases - genetics, Peripheral Nervous System Diseases - immunology, Peripheral Nervous System Diseases - metabolism, Rats, Rats, Sprague-Dawley, Reaction Time - genetics, RNA, Messenger - metabolism, Sciatic Nerve - metabolism, Sciatic Nerve - pathology, Sciatic Nerve - physiopathology, Sciatic Neuropathy - genetics, Sciatic Neuropathy - immunology, Sciatic Neuropathy - metabolism, Traumas. Diseases due to physical agents, Up-Regulation - genetics, Wallerian Degeneration - genetics, Wallerian Degeneration - immunology, Wallerian Degeneration - metabolism